Leica – Confocal Microscopes

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  • Confocal Microscopes Brochure – Leica

Stellaris Confocal Microscopes can be combined with all Leica modalities, including FLIM, STED, DLS, and CRS. With the STELLARIS confocal platform, we have re-imagined confocal microscopy to get you closer to the truth.

  • Follow molecular interactions with FLIM-FRET
  • Monitor subtle and fast changes with biosensors
  • More reliable and sensitive metabolic imaging
  • Fluorophore separation beyond the spectral options
  • STELLARIS 8 FALCON for fast lifetime imaging
  • All-in-one multimodal imaging

Technical Specifications

STELLARIS 5 delivers the very best of the Leica confocal platform. It is a powerful core system with integrated WLL, Acousto-Optical Beam Splitter (AOBS), and Power HyD S detectors.STELLARIS 8 offers all the benefits of the core system plus added functionality, thanks to an extended-spectrum WLL, specialized detector options, and access to dedicated modalities.
Detector
Power HyD Sincludedincluded
Power HyD X, HyD Roptionaloptional
Lasers
WLL 485 – 685 nm (+ optional NIR Extension 685-790 nm)not availablenot available
WLL 440 – 790 nmincludedincluded
Back-to-light WLL serviceoptionaloptional
Features
TauSenseoptionalincluded
ImageCompassincludedincluded
LIGHTNINGincludedincluded
Dynamic signal enhancementincludedincluded
AiviaMotionoptionaloptional
Navigatorincluded
Modalities
STEDnot availableoptional
DIVEnot availableoptional
FALCONincludedoptional
DLSoptionaloptional
CRSnot availableoptional

Key Features

The Power HyD detector family provides higher photon detection efficiency (PDE) extremely low dark noise, and sensitive spectral detection from 410 to 850 nm.

  • Enhanced image quality: STELLARIS provides an ideal combination of brightness, resolution and contrast.
  • Complete spectral freedom: Our next-generation White Light Lasers allow you to simultaneously use up to 8 single excitation lines from across the spectrum. Image more fluorophore combinations than with any other confocal platform and use more labels in parallel, expanding your options into the NIR range.
  • Gentle Live Cell Imaging: Preserve your sample’s integrity and image for longer periods of time through efficient signal acquisition at the lowest required levels of illumination.
  • STELLARIS’ unique TauSense technology enables the extraction of an additional layer of information from each sample, amplifying the scientific impact of your research.
  • TauSense comprises application-oriented imaging tools based on fluorescence lifetime, allowing exploration of molecular functions within the cellular context.
  • TauContrast grants immediate access to functional information, including metabolic status, pH, and ion concentration.
  • TauGating enhances image quality by eliminating unwanted fluorescence contributions.
  • TauSeparation facilitates the expansion of fluorescent signal combinations in your experiment beyond spectral options.
  • TauInteraction offers straightforward detection and quantification of molecular interactions, such as protein-protein interaction.
  • Streamlined setup and acquisition: ImageCompass, the STELLARIS smart user interface, provides users with an easy and intuitive way to set up even the most complex experiments with just a few clicks.
  • Simply fast: Super image quality in real time and at full speed with the LIGHTNING super-resolution, Dynamic Signal Enhancement (DSE), and the powerful AI feature AiviaMotion.
  • Intuitive User Interface: ImageCompass guides you from the experiment setup through acquisition.
  • Optimize your experiment: Tools like LAS X Navigator are seamlessly integrated for straightforward imaging.
  • Aivia and LAS X, advanced image analysis software solutions from Leica Microsystems, empower you to elevate your microscopy-based research.
  • By integrating STELLARIS with Leica’s AI-driven image analysis software Aivia, you can extract meaningful insights from your microscopy imaging data using a wide range of machine learning and deep learning algorithms.
  • The image depicts maturation states corresponding to distinct pH values, captured through changes in the fluorescence lifetime of the NIR stain using the TauSeparation tool available in TauSense.
  • In this example, four stages are identified from early to late endosomes and lysosomes (cyan, yellow, magenta, red).
  • The individual endosomes are identified and tracked using the Aivia 3D Object Tracking tool (color-coded spheres and traces).
  • Aivia analysis provides relevant parameters for characterizing the endocytic pathway, such as path length, mean track velocity, mean diffusion coefficient, and mean curvature.

How It Works

Leica – Stellaris Confocal Microscopes Platforms

  • Confocal
  • WWL – white light laser
  • Power HyD detector
  • Follow molecular interactions
  • Fluorophore separation
  • Acousto-Optical Beam Splitter

Available Models

Introduction To LAS X For STELLARIS

Brand

Leica Microsystems

Leica Microsystems develops and manufactures microscopes and scientific instruments for the analysis of microstructures and nanostructures.

Widely recognized for optical precision and innovative technology, the company is one of the market leaders in compound and stereo microscopy, digital microscopy, confocal laser scanning and super-resolution microscopy with related imaging systems, electron microscopy sample preparation, and surgical microscopy.

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