Leica – Confocal Microscopes

Stellaris Confocal Microscopes can be combined with all Leica modalities, including FLIM, STED, DLS, and CRS. With the STELLARIS confocal platform, we have re-imagined confocal microscopy to get you closer to the truth.

  • Follow molecular interactions with FLIM-FRET
  • Monitor subtle and fast changes with biosensors
  • More reliable and sensitive metabolic imaging
  • Fluorophore separation beyond the spectral options
  • STELLARIS 8 FALCON for fast lifetime imaging
  • All-in-one multimodal imaging

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Overview

Features

Specifications

Technical Specifications

STELLARIS 5 delivers the very best of the Leica confocal platform. It is a powerful core system with integrated WLL, Acousto-Optical Beam Splitter (AOBS), and Power HyD S detectors.STELLARIS 8 offers all the benefits of the core system plus added functionality, thanks to an extended-spectrum WLL, specialized detector options, and access to dedicated modalities.
Detector
Power HyD Sincludedincluded
Power HyD X, HyD Roptionaloptional
Lasers
WLL 485 – 685 nm (+ optional NIR Extension 685-790 nm)not availablenot available
WLL 440 – 790 nmincludedincluded
Back-to-light WLL serviceoptionaloptional
Features
TauSenseoptionalincluded
ImageCompassincludedincluded
LIGHTNINGincludedincluded
Dynamic signal enhancementincludedincluded
AiviaMotionoptionaloptional
Navigatorincluded
Modalities
STEDnot availableoptional
DIVEnot availableoptional
FALCONincludedoptional
DLSoptionaloptional
CRSnot availableoptional

Key Features

  • Follow molecular interactions with FLIM-FRET
  • Monitor subtle and fast changes with biosensors
  • More reliable and sensitive metabolic imaging
  • Fluorophore separation beyond the spectral options
  • STELLARIS 8 FALCON for fast lifetime imaging
  • All-in-one multimodal imaging

The Power HyD detector family provides higher photon detection efficiency (PDE) extremely low dark noise, and sensitive spectral detection from 410 to 850 nm.

  • Enhanced image quality: STELLARIS provides an ideal combination of brightness, resolution and contrast.
  • Complete spectral freedom: Our next-generation White Light Lasers allow you to simultaneously use up to 8 single excitation lines from across the spectrum. Image more fluorophore combinations than with any other confocal platform and use more labels in parallel, expanding your options into the NIR range.
  • Gentle Live Cell Imaging: Preserve your sample’s integrity and image for longer periods of time through efficient signal acquisition at the lowest required levels of illumination.
  • STELLARIS’ unique TauSense technology enables the extraction of an additional layer of information from each sample, amplifying the scientific impact of your research.
  • TauSense comprises application-oriented imaging tools based on fluorescence lifetime, allowing exploration of molecular functions within the cellular context.
  • TauContrast grants immediate access to functional information, including metabolic status, pH, and ion concentration.
  • TauGating enhances image quality by eliminating unwanted fluorescence contributions.
  • TauSeparation facilitates the expansion of fluorescent signal combinations in your experiment beyond spectral options.
  • TauInteraction offers straightforward detection and quantification of molecular interactions, such as protein-protein interaction.
  • Streamlined setup and acquisition: ImageCompass, the STELLARIS smart user interface, provides users with an easy and intuitive way to set up even the most complex experiments with just a few clicks.
  • Simply fast: Super image quality in real time and at full speed with the LIGHTNING super-resolution, Dynamic Signal Enhancement (DSE), and the powerful AI feature AiviaMotion.
  • Intuitive User Interface: ImageCompass guides you from the experiment setup through acquisition.
  • Optimize your experiment: Tools like LAS X Navigator are seamlessly integrated for straightforward imaging.
  • Aivia and LAS X, advanced image analysis software solutions from Leica Microsystems, empower you to elevate your microscopy-based research.
  • By integrating STELLARIS with Leica’s AI-driven image analysis software Aivia, you can extract meaningful insights from your microscopy imaging data using a wide range of machine learning and deep learning algorithms.
  • The image depicts maturation states corresponding to distinct pH values, captured through changes in the fluorescence lifetime of the NIR stain using the TauSeparation tool available in TauSense.
  • In this example, four stages are identified from early to late endosomes and lysosomes (cyan, yellow, magenta, red).
  • The individual endosomes are identified and tracked using the Aivia 3D Object Tracking tool (color-coded spheres and traces).
  • Aivia analysis provides relevant parameters for characterizing the endocytic pathway, such as path length, mean track velocity, mean diffusion coefficient, and mean curvature.

How It Works

Leica – Stellaris Confocal Microscopes Platforms

  • Confocal
  • WWL – white light laser
  • Power HyD detector
  • Follow molecular interactions
  • Fluorophore separation
  • Acousto-Optical Beam Splitter

Available Models

Introduction To LAS X For STELLARIS

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  • Confocal Microscopes Brochure – Leica

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