ATTO – Western Blotting
Western blotting is the basic experimental method to specifically and high-sensitively detect desired component from protein sample. It is roughly divided into 2 types; “Semi Dry type” and “Wet type (Tank type)”. ATTO, recommend and provide Semi Dry type blotting system based on the following merits.
- Less Amount of Blotting Solution
- Excellent Operability
- Small Heating Value
- Short Operating Time
- More Effective With Optimized Blotting Solution
Technical Specifications
| Model | WSE-4115 | WSE-4125 | WSE-4025 | WSE-4045 |
|---|---|---|---|---|
| Electrode size | 95 (W) x 98 (D) mm | 205 (W) x 100 (D) mm | 205mm(W)×100mm（D） | 205 (W) x 200 (D) mm |
| Distance between electrodes | Min: 3 mm. Max: 10 mm | Min: 3 mm, Max: 15 mm | Min: 3 mm, Max: 15 mm | Min: 3 mm. Max: 15 mm |
| Available number of gel for blotting | 1 mini gel | 2 mini gels | Maximum of 2 mini gels | 4 mini gels |
| Method | Vertical (Semi-dry blotting) | Vertical (Semi-dry blotting) | Horizontal (semi-dry blotting) | Vertical (Semi-dry blotting) |
| Blotting solution volume | 50-100 mL (depending on the size and number of gel) | 100-200 mL (depending on the size and number of gel) | 100-200 mL (depending on the size and number of gel) | 100-400 mL (depending on the size and number of gel) |
| Electrode polarity | Top: Cathode / Bottom: Anode | Top: Cathode / Bottom: Anode | Top: Cathode / Bottom: Anode | Top: Cathode / Bottom: Anode |
| Power supply | STD mode: 12V constant voltage RAPID mode: 24V constant voltage Timer: 1-200 min (display the remaining time) | STD mode: 12V constant voltage RAPID mode: 24V constant voltage Timer: 1-250 min (display the remaining time) Power consumption: 26W | ||
| AC adapter | Input: 100-240V (#10%) 50/60Hz (‡5%) Outout: DC24V/1.5A | Input: 100-240V (#10%) 50/60HZ (‡5%) Output: DC24V/1.5A | ||
| Material | Main unit: Acrylic Anode: Platinized titanium Cathode: Corrosion-resistant stainless steel Power supply case: ABS resin | Main unit: Acrylic Anode: Platinized titanium Cathode: Corrosion-resistant stainless steel | Main Body: Acrylic Upper Electrode: Chemical-resistant Stainless Steel Lower: Platinum-plated Titanium | Main unit: Acrylic Anode: Platinized titanium Cathode: Corrosion-resistant stainless steel |
| Dimension / Weight | Main unit (without projection): 170 (W) x 120 (D) x 107 (H) mm, 0.77 kg AC adapter(without projection): 36 (W) x 95 (D) x 31 (H) mm, 0.14 kg | Main unit (without projection): 246 (W) x 186 (D) x 114 (H) mm, 2.1 kg AC adapter(without projection): 36 (W) x 95 (D) x 31 (H) mm, 0.14 kg | 246mm (W) x 145mm (D) x 92mm (H) (excluding protrusions) 1.8kg (excluding lead wire) | 246 (W) x 235 (D) x 92 (H) mm (Main unit without projection) 3.0 kg (except wiring) |
| Components | WSE-4115 main unit 1 ea AC adapter 1 ea Extension cable 1 ea Power lead wire with safety terminal and reverse polarity prevention 1 ea Roller 1 ea | WSE-4125 main unit 1 ea AC adapter 1 ea Extension cable 1 ea Power lead wire with safety terminal and reverse polarity prevention 1 ea Roller ea | WSE-4025 Main Body: 1 unit Lead Wire with Reverse Connection Prevention Mechanism and Safety Terminal: 1 piece Roller Fixture: 1 piece Instruction Manual: 1 booklet | WSE-4045 main unit 1 ea Power lead wire with safety terminal and reverse polarity prevention 1 ea Roller 1 ea |
Available Models
For use in 1 gel of compact size/ mini gel size
Key Features
- Semi-dry blotting system built-in power supply part
- Save space
- Output mode can be selected from 2 modes
1. STD (Standard)：
Constant voltage 12 V
Standard transferring time：30-60 min (Timer: 1-200 min)
2. RAPID：
Constant voltage 24 V
Standard transferring time：10-30 min (Timer: 1-200 min)
- Easily and certainly setting membrane, gel and filter
- Upper electrode includes springs so clear blotting is possible
For use in 2 gels of mini gel size
Key Features
- Semi-dry blotting system with a built-in power supply offering;
Fast blotting is available, space-saving, easy operation, high reproducibility - Improved Electrode plate setting enabled;
Uniform and firm contact of gels and membranes, and fast blotting - Electrode plate size: 205mm x 100mm
- Two mini gels can be transferred simultaneously
- It takes 10min minimum in fast blotting mode, and 30 to 60min in standard blotting mode
- Including a hand roller for setting gels/membranes/filter papers
- About 1/10 of transfer buffer of wet type (tank) is just enough
- Chemically resistant electrode plate can be washed after use to be kept clean
Semi-dry blotting for transferring proteins from 2 mini gels
Key Features
- Improved Electrode plate setting enabled;
uniform and firm contact of gels and membranes; fast blotting - Electrode plate size: 205 mm X 100 mm
- For use in 2 mini gels
- Uniform transfer efficiency with improved contact of gels and membranes
- Available for standard and fast blotting system
Standard blotting condition: Constant voltage, 12 V, 30-60 min
Fast blotting condition: Constant voltage, 24 V, 10 min - Rapid semi-dry blotting is possible with AE-1465 EzFastBlot (10-30min)
- Blotting high molecular protein is possible with WSE-7210 EzFastBlot HMW (30-60min)
- Including a hand roller for setting gels/membranes/filter papers
- About 1/10 of transfer buffer of wet type (tank) is just enough
- Chemically resistant electrode plate can be washed after use to be kept clean
Semi-dry blotting for transferring proteins from 2 mini gels
Key Features
- Improved Electrode plate setting enabled;
- uniform and firm contact of gels and membranes; fast blotting
- Electrode plate size: 205×200 mm
- For use in 4 mini gels (8 x 9cm), or Larger size gel blotting is available
- Rapid semi-dry blotting is possible with AE-1465 EzFastBlot (10-30min)
- Blotting high molecular protein is possible with WSE-7210 EzFastBlot HMW (30-60min)
- Expert roller included for easy setting layout (gel, membrane, filter paper)
- 1/10 of transfer buffer is enough of a wet blot
- Electrode for drug resistance: washable system
Western Blotting Operation Video
Key Industries
- Biopharmaceutical and Biotechnology
- Education & Academics
- Medical
- Pharmaceutical
Brand
ATTO Corporation is a scientific instrument developer and manufacturer of protein/nucleic acid research. Long been providing more than 60 years for Polyacrylamide Gel Electrophoresis Devices, PAGEL (precast gel), Western blot solutions, Peristaltic Pumps, Printgraph (High-end Gel Documentation System), Luminograph series (Imaging System for bio/chemiluminescence detection), etc. Their products have been used in many laboratories in Japan and other countries.
