Clear 3D Cell Culture Models With Minimal Changes to Morphology and Without Compromising Sensitivity

Despite the growing relevance of 3D cell culture as a research model, imaging techniques used to characterize these models are highly limited. Due to the thickness and opacity of 3D cellular structures, most current imaging technologies cannot penetrate to the center of the tissues, resulting in only the outer 2 to 3 layers of cells being detected. This causes the dark centers often seen in images of 3D cell culture models, which proves highly problematic for accurate analysis as these outer cells are most exposed to compounds, nutrients and oxygen and thus do not reflect the entire cell population.

Corning now offers 3D Clear Tissue Clearing reagent that can be used in a tissue clearing technique, designed specifically to support imaging for 3D cell culture models and plate-based high throughput processing. When paired with fluorescent labeling (e.g., fluorescent protein, immunofluorescence, chemical dyes) and high content confocal microscopy, Corning 3D Clear reagent allows for complete 3D cell culture model characterization and more accurate drug screening.

Growth Promotion and Cytotoxicity
Corning classical media products undergo a variety of tests to determine functionality and toxicity using growth-promotion analysis. Product performance is measured by harvest-to-plant ratios or fold increase according to predetermined specifications.

Physiochemical Properties
Liquid and powder classical media products are tested to determine pH and osmolality. Classical media powders are also tested for residual moisture. The pH and osmolality tests are performed according to specific procedures in which all equipment is calibrated using standards traceable to the National Institute of Standards and Technology.

Biological Tests
Quality release testing of Corning liquid classical media products assures low levels of endotoxin, mycoplasma, and an absence of contamination.

Endotoxin
Classical media products are tested for bacterial endotoxin using the Limulus Amoebocyte Lysate (LAL) chromogenic assay. For release, each lot of standard liquid classical media must have an endotoxin level of <0.25 EU/mL. Classical media powder products must have an endotoxin level of <0.25 EU/mL when reconstituted to formula weight with low endotoxin water.

Sterility
All Corning liquid classical media products are tested for sterility.

Mycoplasma
Liquid classical media products are tested for mycoplasma using a large-volume method.

Corning water is available in a variety of sizes, and tested to specifications which may include USP and/or EP monograph requirements.

PYREX® flat bottom boiling flasks have long necks with tooled mouths and take a No. 2 rubber stopper. They are manufactured from No. 7740 borosilicate glass. Wall thicknesses are carefully controlled to provide the proper balance between thermal expansion and mechanical strength for greater shock resistance. Their excellent chemical durability results in contamination-free results and long life under the toughest lab conditions. The normal working temperature for PYREX® boiling flasks is 230°C.

Nhóm cốc thuỷ tinh thấp thành có vòi, được sản xuất với độ dày thành đồng nhất với sự cân bằng tối ưu, khả năng chống sốc nhiệt và đô bền cơ học. Các cốc thuỷ tinh có dung tích từ 250 đến 4000 mL và đều có điểm đánh dấu cực lớn. Riêng cỡ 10 mL không chia vạch đo.

PYREX® round bottom boiling flasks have short ring necks and take a No. 5 rubber stopper. These ring neck boiling flasks are precisely tooled for uniform stopper fit. These flasks are used in applications requiring rigid stoppering and as wash bottles in routine lab washing. They are manufactured from No. 7740 borosilicate glass. Wall thicknesses are carefully controlled to provide the proper balance between thermal expansion and mechanical strength for greater shock resistance. Their excellent chemical durability results in contamination-free results and long life under the toughest lab conditions. The normal working temperature for PYREX® boiling flasks is 230°C.

Bovine calf serum is a cost-effective alternative to Fetal Bovine Serum (FBS). It is often used with robust cell lines when large quantities of serum are needed such as in the manufacturing of biological drugs and vaccines. Bovine calf serum is derived from calves that are generally less than 22 weeks old. It has very high levels of transferrin, which allows for iron fortification to levels of more than three times greater than normal FBS. Iron is essential for cell respiration and metabolism and for some cell lines, makes up for the lower levels of growth factors and other metabolites found in calf serum when compared to FBS.

Like ampicillin, carbenicillin interferes with cell-wall synthesis of actively growing cells.