NanoCellect – Wolf G2 Cell Sorter
Healthy Cells. Broad Capabilities.Better Science.
The NanoCellect Wolf G2 cell sorter is a powerful tool used for a variety of applications, including antibody discovery, single cell sorting and plating, genomics, single cell RNA sequencing, plant biology, gene editing, cell line development, and discovering microorganisms in ocean or pond water. The Wolf G2 is designed to gently sort happy, healthy cells using a sterile cartridge-based microfluidics system. The Wolf G2 cell sorter is ideal for tasks like cell line development and sorting plant protoplasts, as well as for monoclonal outgrowth of iPSCs and microalgae.
Technical Specifications
| Instrument Specifications | |
| Wolf Dimensions | 37.6H x 45.8W x 34.5D cm |
| Wolf Weight | 24.5kg |
| Wolf Electrical | AC Input: 100-240V, 50/60Hz, 0.5A |
| N1 Dimensions | 21.4 x 16.5 x 20.9 cm |
| N1 Weight | 2.5 kg |
| N1 Electrical | DC Input: 24V 1A |
| Performance | |
| Scatter sensitivity | < 1.5 μm by FSC or BSC |
| Scatter Resolution | Resolves lymphocytes, monocytes, and granulocytes |
| Fluorescence sensitivity | < 200 MESF FITC (using 488 nm laser) < 250 MESF PE (using 561 nm laser) |
| Fluorescence Resolution | 8 peak separation with SPHERO™️ Rainbow Calibration Particles |
| Analysis Speed | > 5,000 events/second |
| Sorting | 1- and 2-way |
| Back-to-back Sorting Speed | 200 events per second |
| Absolute Counts | Yes |
| Volumetric Analysis | Yes |
| Warm-up Time | Less than 1 minute |
| Sorting Purity | Up to 99% purity |
| Sorting Output | 1.5 mL and 5.0 mL, 96- and 384-well plates |
Key Features
Healthy Cells
At < 2 psi, the Wolf G2 are gentler than any conventional cell sorters, enabling healthier cells post-sort, especially for engineered lines, primary cells, and stem cells.
High Sensitivity and Resolution
All laser configurations afford < 250 MESF sensitivity, along with forward and back scatter, providing as low as 1 μm resolution.
Compact and at your bench
At 2 cubic feet, NanoCellect’s benchmark for access and performance allows every lab for the flexibility to do analysis and sorting into tubes or 96- and 384-well plates.
Simple and Intuitive
Intuitive software, fixed optics, no fluidics cart and less than one minute clean-up time.
Contaminant-free and Biohazard-free
Disposable, aerosol-free microfluidic cartridge allows for sterile sorting that protects the sample from the environment and scientist from the sample.
Expanding the Wolf’s Capabilities
With two lasers and up to nine fluorescent channels, the Wolf G2 aligns with a broad set of research applications and experiments. Three different laser configurations allows options specific to your needs.
Brand
NanoCellect is committed to empowering every scientist to make discoveries one cell at a time, with modern and simple technologies for to advance cell-based assays that are affordable, compact, and easy-to-use. Our microfluidic flow cytometry platforms enable biomedical scientists to analyze and sort cells required for drug discovery, single cell-omics, cloning, and basic research.